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Pi minimal Screen
The Pi-Screens were developed at the MRC Laboratory of Molecular Biology (Cambridge, UK) for efficient crystallization screening of soluble proteins (Pi-minimal Screen) and integral membrane proteins (Pi-PEG Screen). The approach is based on incomplete factorial design.

The unique formulation was generated following a strategy named Pi sampling [1] in order to create novel combinations of precipitants, buffers and additives across a standard 96-condition plate layout. Thus, the diversity amongst the crystallization conditions is ideal for initial screening.

The Pi-minimal Screen includes 36 components, i.e. 12 precipitants, 12 buffers systems and 12 salts. Buffers employed in the Pi-minimal screen are buffer systems (acid-base pairs, e.g. HEPES and HEPES sodium salt). Consequently, pH can be adjusted by mixing 2 stock solutions at different ratios during later optimizations.
The efficiency of the Pi-minimal Screen was demonstrated by the crystallization of 10 proteins before its commercialization [1].

The Pi-PEG Screen includes various polyethylene glycol mixtures, additives and buffers covering a pH range from 4,0 9,5 and hence is suitable for integral membrane proteins as well as for soluble proteins.
The efficiency of the Pi-PEG screen was demonstrated by the crystallization of a G-protein coupled receptor (GPCR) when quality crystals could not be produced with other commercially available screens [1].

Bulk 4 x 24 screening solutions in 10 ml aliquots
HTS 96 screening solutions delivered in a deep-well block, 1.7 ml per well

Individual Conditions of all screens are available in 10 ml as well as 100 ml volumes. Please follow this link.

Product Cat. No. Amount Price (EUR) Buy / Note Downloads
Pi-minimal Screen CS-127 4 x 24 solutions
(10 ml each)
708,00    Add this product to your notepad      
Pi-PEG Screen CS-128 4 x 24 solutions
(10 ml each)
708,00    Add this product to your notepad      
Pi-minimal Screen HTS CS-211L 96 solutions
(1,7 ml each)
354,00    Add this product to your notepad      
Pi-PEG Screen HTS CS-212L 96 solutions
(1,7 ml each)
354,00    Add this product to your notepad      


[1] Gorrec et al. (2011) Pi sampling: a methodical and flexible approach to initial macromolecular crystallization screening. Acta Cryst. D67:463.

Available online at http://journals.iucr.org/d/issues/2011/05/00/bw5391/index.html

Selected Recent Literature Citations of Pi-Screens

  • Omari et al. (2014) Pushing the limits of sulfur SAD phasing: de novo structure solution of the N-terminal domain of the ectodomain of HCV E1. Acta Cryst. D 70:2197.

Please contact xtals@jenabioscience.com with questions or inquiries.