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BseB I
Isoschizomers: BstN I, BstO I, Bst2U I, Mva I
Neoschizomers: Ajn I, EcoR II, Psp6 I, PspG I | CC↓(A/T)GG | |
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S pack |
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EN-108S |
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4500 Units |
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25,00 |
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L pack |
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EN-108L |
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22500 Units |
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100,00 |
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| | Source: Bacillus staerothermophilus.
Buffer supplied: 10x B2 (incl. 10x BSA).
Substrate for unit definition: λ DNA (70 sites).
Reaction conditions: 50 mM NaCl, 10 mM Tris-HCl (pH @ 7.9 @ 25°C), 10 mM MgCl2, 1 mM dithiothreitol, 100 µg/ml BSA. Incubate at 60°C.
Storage buffer: 50 mM KCl, 10 mM Tris-HCl (pH 7.4), 0.1 mM EDTA, 1 mM dithiothreitol, 200 µg/ml BSA and 50 % glycerol. Store at -20°C. | | Ligation and recutting: After ten-fold overdigestion with BseB I, <50 % of the DNA fragments can be ligated.
Star activity: Low salt concentration or large excess of enzyme results in the appearance of star activity.
Note: BseB I-cut DNA is difficult to ligate with T4 DNA Ligase. Ligation is enhanced in the presence of 15 % PEG4000.
Heat inactivation: No. | | |
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