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  Standard PCR - Supplements
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Ready-to-Use Mixes / direct gel loading   |   Ready-to-Use Mixes   |   Core Kits   |   Thermophilic Polymerases   |   Lyophilisates   |   dNTP Mixes   |   dNTP Bundles   |   Supplements
 

  
Standard PCR Supplements

Convenient tools designed to save you valuable time at the bench and yielding superior results and performance. The Kits are recommended to facilitate the amplification of GC-rich structures, to enhance the yield and to serve as an internal lab standard.

Standard PCR - Product Selection Guide


 Gel Loading Buffer with DNA Stain
 Loading buffer for agarose or polyacrylamide gels with EvaGreenTM fluorescent DNA stain
 
Product Cat. No. Amount Price (EUR) Buy  
Blue PCR-255-bl 5x1 ml 35,00  
Green PCR-255-gr 5x1 ml 35,00
Orange PCR-255-or 5x1 ml 35,00
Store at 2-8°C in the dark

6x concentration
Gel Loading Buffers with DNA Stain are formulated to facilitate loading of DNA samples into the wells of agarose and polyacrylamide gels. The loading buffers contain EvaGreenTM DNA Stain a fluorescent DNA intercalator dye specially developed for DNA analysis applications. Its high quantum yield and excellent stability makes it the ideal fluorophore for DNA staining applications and a superior replacement for the widely used dyes Ethidium Bromide or SYBR® Green.
The buffers contain tracking dyes as indicators for DNA fragment migration.
The Gel Loading Buffers provide highest convenience during routine handling and avoid commonly used gel staining procedures with Ethidium Bromide or SYBR® Green.
 Gel Loading Buffer
 Loading buffer for agarose or polyacrylamide gels
 
Product Cat. No. Amount Price (EUR) Buy  
Blue PCR-254-bl 5x1 ml 25,00  
Green PCR-254-gr 5x1 ml 25,00
Orange PCR-254-or 5x1 ml 25,00
Store at 2-8°C

6x concentration
Gel Loading Buffers are formulated to facilitate loading of DNA containing samples into the wells of agarose and polyacrylamide gels.
Blue Gel Loading Buffer contains bromophenol blue and xylene cyanol FF as tracking dyes and indicator for DNA fragment migration. It is recommended for loading of fragment larger than 500 bp.
Green Gel Loading Buffer contains orange G and xylene cyanol FF as tracking dyes and is recommended for loading of fragment from 100 to 2000 bp.
Orange Gel Loading Buffer contains orange G and is recommended for loading of fragment smaller than 500 bp.
 EvaGreenTM Fluorescent Gel Stain
 Staining dye for DNA gel electrophoresis
 
Product Cat. No. Amount Price (EUR) Buy  
Kit PCR-256 100 µl 40,00  
Store at -20°C in the dark

100x concentration
EvaGreenTM Fluorescent Gel Stain is a superior DNA staining dye for DNA analysis in gel electrophoresis applications. Upon binding to DNA, the non-fluorescent dye becomes highly fluorescent allowing the detection of lowest DNA amounts. It is extremely stable both thermally and hydrolytically, providing convenience during routine handling.
 PCR Control Kit
 Amplification of a beta-actin gene fragment from human genomic DNA
 
Product Cat. No. Amount Price (EUR) Buy  
Kit PCR-253 500 reactions 50,00  
Store at -20°C

ß-actin forward Primer
ß-actin reverse Primer
Human genomic DNA (male)
PCR Control Kit is designed for quality and benchmark tests, as positive control or internal lab standard for Taq DNA polymerases. The kit contains a forward / reverse primer-pair designed to amplify a 295 bp long fragment of the β-actin gene from human genomic DNA.
 dNTP Mix GCamplifier
 Modified dNTP Mix for amplification of GC-rich sequences
 
Product Cat. No. Amount Price (EUR) Buy  
Kit PCR-257 100 µl 80,00  
Store at -20°C

10 mM each dNTP
dNTP PCR Mix GCamplifier is specially designed for amplification of GC-rich DNA templates. The kit contains a modified dGTP analog that significantly reduces the stability of GC-rich sequences thereby enhancing PCR efficiency. The dNTP PCR Mix GCamplifier is used instead of a standard dNTP mix.
 PCR Additives Kit
 Stabilizer and enhancer
 
Product Cat. No. Amount Price (EUR)