Products & Ordering
User Login
Order Information
"S" Enzymes - Sty I| Sty I Isoschizomers: BssT1 I, Eco130 I, EcoT14 I, Erh I |
C↓C(A/T)(T/A)GG | ||||
| Product | Cat. No. | Amount | Price (EUR) | Buy / Note | |
|---|---|---|---|---|---|
| S pack | EN-141S | 6000 Units | 25,00 | 
 |
 |
| L pack | EN-141L | 30000 Units | 100,00 |
|
|
Recognition Sequence:
Source: E.coli WA921/pST27 hsd+.
Buffer supplied: 10x Reaction Buffer B3.
Storage buffer: 50 mM KCl, 10 mM Tris-HCl (pH 7.4), 0.1 mM EDTA,
1 mM dithiothreitol, 200 µg/ml BSA and 50 % [v/v] glycerol.
Store at -20°C.
Reaction conditions: 100 mM NaCl, 50 mM Tris-HCl (pH 7.9 at 25°C), 10 mM MgCl2, 1 mM dithiothreitol and 100 µg/ml BSA.
Incubate at 37°C.
5
'
-
C
|
C
W
W
G
G
-
3
'
3
'
-
G
G
W
W
C
|
C
-
5
'
Source: E.coli WA921/pST27 hsd+.
Buffer supplied: 10x Reaction Buffer B3.
Storage buffer: 50 mM KCl, 10 mM Tris-HCl (pH 7.4), 0.1 mM EDTA,
1 mM dithiothreitol, 200 µg/ml BSA and 50 % [v/v] glycerol.
Store at -20°C.
Reaction conditions: 100 mM NaCl, 50 mM Tris-HCl (pH 7.9 at 25°C), 10 mM MgCl2, 1 mM dithiothreitol and 100 µg/ml BSA.
Incubate at 37°C.
Heat inactivation: 20 minutes at 65°C.
Ligation and recutting: After ten-fold overdigestion with Sty I, >98 % of the DNA fragments can be ligated and recut with this enzyme.
DNA Methylation: No Inhibition: dam, dcm, CpG
Unit Definition: One unit is the amount of enzyme required to completely digest 1 µg of Lambda DNA (10 sites) in 1 hour in a total reaction volume of 50 µl. Enzyme activity was determined in the recommended reaction buffer.
Ligation and recutting: After ten-fold overdigestion with Sty I, >98 % of the DNA fragments can be ligated and recut with this enzyme.
DNA Methylation: No Inhibition: dam, dcm, CpG
Unit Definition: One unit is the amount of enzyme required to completely digest 1 µg of Lambda DNA (10 sites) in 1 hour in a total reaction volume of 50 µl. Enzyme activity was determined in the recommended reaction buffer.