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"S" Enzymes - SseB I| SseB I Isoschizomers: Aat I, Eco147 I, Pce I, Stu I |
AGG↓CCT | ||||
| Product | Cat. No. | Amount | Price (EUR) | Buy / Note | |
|---|---|---|---|---|---|
| S pack | EN-138S | 1500 Units | 25,00 | 
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| L pack | EN-138L | 7500 Units | 100,00 |
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Recognition Sequence:
Source: Streptomyces species.
Buffer supplied: 10x Reaction Buffer B3.
Storage buffer: 50 mM KCl, 10 mM Tris-HCl (pH 7.9), 0.1 mM EDTA,
1 mM dithiothreitol, 200 µg/ml BSA and 50 % [v/v] glycerol.
Store at -20°C.
Reaction conditions: 100 mM NaCl, 50 mM Tris-HCl (pH 7.9 at 25°C), 10 mM MgCl2, 1 mM dithiothreitol and 100 µg/ml BSA.
Incubate at 37°C.
5
'
-
A
G
G
|
C
C
T
-
3
'
3
'
-
T
C
C
|
G
G
A
-
5
'
Source: Streptomyces species.
Buffer supplied: 10x Reaction Buffer B3.
Storage buffer: 50 mM KCl, 10 mM Tris-HCl (pH 7.9), 0.1 mM EDTA,
1 mM dithiothreitol, 200 µg/ml BSA and 50 % [v/v] glycerol.
Store at -20°C.
Reaction conditions: 100 mM NaCl, 50 mM Tris-HCl (pH 7.9 at 25°C), 10 mM MgCl2, 1 mM dithiothreitol and 100 µg/ml BSA.
Incubate at 37°C.
Heat inactivation: 20 minutes at 65°C.
Ligation and recutting: After 10-fold overdigestion with SseB I,
>95 % of the DNA fragments can be ligated and recut with this enzyme.
DNA Methylation:
No Inhibition: dam, CpG
Inhibition (Blocked by overlapping): dcm
Unit Definition: One unit is the amount of enzyme required to completely digest 1 µg of Lambda DNA (Hind III digest; 6 sites) in 1 hour in a total reaction volume of 50 µl. Enzyme activity was determined in the recommended reaction buffer.
Ligation and recutting: After 10-fold overdigestion with SseB I,
>95 % of the DNA fragments can be ligated and recut with this enzyme.
DNA Methylation:
No Inhibition: dam, CpG
Inhibition (Blocked by overlapping): dcm
Unit Definition: One unit is the amount of enzyme required to completely digest 1 µg of Lambda DNA (Hind III digest; 6 sites) in 1 hour in a total reaction volume of 50 µl. Enzyme activity was determined in the recommended reaction buffer.