Products & Ordering
User Login
Order Information
"S" Enzymes - SnaB I| SnaB I Isoschizomers: BstSN I, Eco105 I |
TAC↓GTA | ||||
| Product | Cat. No. | Amount | Price (EUR) | Buy / Note | |
|---|---|---|---|---|---|
| S pack | EN-136S | 350 Units | 25,00 | 
 |
 |
| L pack | EN-136L | 1750 Units | 100,00 |
|
|
Recognition Sequence:
Source: Sphaerotilus natans.
Buffer supplied: 10x Reaction Buffer SnaB I.
Storage buffer: 50 mM KCl, 10 mM Tris-HCl (pH 7.4), 0.1 mM EDTA,
1 mM dithiothreitol, 200 µg/ml BSA and 50 % [v/v] glycerol.
Store at -20°C.
Reaction conditions: 10 mM Bis Tris Propane-HCI (pH 7.0 at 25°C),
10 mM MgCl2, 1 mM dithiothreitol and 100 µg/ml BSA.
Incubate at 37°C.
5
'
-
T
A
C
|
G
T
A
-
3
'
3
'
-
A
T
G
|
C
A
T
-
5
'
Source: Sphaerotilus natans.
Buffer supplied: 10x Reaction Buffer SnaB I.
Storage buffer: 50 mM KCl, 10 mM Tris-HCl (pH 7.4), 0.1 mM EDTA,
1 mM dithiothreitol, 200 µg/ml BSA and 50 % [v/v] glycerol.
Store at -20°C.
Reaction conditions: 10 mM Bis Tris Propane-HCI (pH 7.0 at 25°C),
10 mM MgCl2, 1 mM dithiothreitol and 100 µg/ml BSA.
Incubate at 37°C.
Heat inactivation: 20 minutes at 80°C.
Ligation and recutting: After ten-fold overdigestion with SnaB I,
>98 % of the DNA fragments can be ligated and recut with this enzyme.
Star activity: Star activity is observed at a greater than 160-fold overdigestion with SnaB I.
DNA Methylation:
No Inhibition: dam, dcm
Inhibition (Blocked): CpG
Unit Definition: One unit is the amount of enzyme required to completely digest 1 µg of Lambda DNA (EcoR I digest; 1 site) in 1 hour in a total reaction volume of 50 µl. Enzyme activity was determined in the recommended reaction buffer.
Ligation and recutting: After ten-fold overdigestion with SnaB I,
>98 % of the DNA fragments can be ligated and recut with this enzyme.
Star activity: Star activity is observed at a greater than 160-fold overdigestion with SnaB I.
DNA Methylation:
No Inhibition: dam, dcm
Inhibition (Blocked): CpG
Unit Definition: One unit is the amount of enzyme required to completely digest 1 µg of Lambda DNA (EcoR I digest; 1 site) in 1 hour in a total reaction volume of 50 µl. Enzyme activity was determined in the recommended reaction buffer.