 | Source: Sphaerotilus natans.
Buffer supplied: 10x SnaB I and 10x BSA.
Substrate for unit definition: λ DNA, EcoR I digest (1 site).
Reaction conditions: 10 mM Bis Tris Propane-HCI (pH 7.0 @ 25°C), 10 mM MgCl2, 1 mM dithiothreitol, 100 µg/ml BSA. Incubate at 37°C. | | Storage buffer: 50 mM KCl, 10 mM Tris-HCl (pH 7.4), 0.1 mM EDTA, 1 mM dithiothreitol, 200 µg/ml BSA and 50 % glycerol. Store at -20°C.
Ligation and recutting: After ten-fold overdigestion with SnaB I, >98 % of the DNA fragments can be ligated and recut with this enzyme.
Star activity: Star activity is observed at a greater than 160-fold overdigestion with SnaB I.
Heat inactivation: 80°C for 20 minutes. | |
