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SgrB I
Isoschizomers: Cfr42 I, Ksp I, Sac II, Sfr303 I | CCGC↓GG | |
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Cat. No. |
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Amount |
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Price (EUR) |
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S pack |
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EN-133S |
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1600 Units |
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25,00 |
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L pack |
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EN-133L |
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8000 Units |
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100,00 |
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| | Source: Streptomyces griseus.
Buffer supplied: 10x B1* and 10x BSA.
Substrate for unit definition: λ DNA, Hind III digest (4 sites).
Reaction conditions: 10 mM Tris-HCl (pH 7.9@ 25°C), 10 mM MgCl2, 1 mM dithiothreitol, 0.1 % Triton X-100, 100 µg/ml BSA. Incubate at 37°C. | | Storage buffer: 50 mM NaCl, 10 mM Tris-HCl (pH 7.4), 0.1 mM EDTA, 1 mM dithiothreitol, 200 µg/ml BSA and 50 % glycerol. Store at -20°C.
Ligation and recutting: After ten-fold overdigestion with SgrB I, >98 % of the DNA fragments can be ligated and recut with this enzyme.
Note: Particular sites in λ and ΦX174 DNAs are difficult to cleave with SgrB I, as well as with its prototype Sac II. Blocked by overlapping dcm methylation.
Heat inactivation: 65°C for 20 minutes. | | |
Note: BSA is not included into the supplied reaction buffers and should be added separately from the supplied stock solution
(1 mg/ml).
* Buffer contains Triton X-100 to ensure optimal enzyme activity.
Please contact enzymes@jenabioscience.com for more information.
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