|
|
 |
|
| |
Sau3A I
Isoschizomers: BfuC I, Bsp143 I, BstEN II, BstMB I, Dpn II, Kzo9 II, NdeI I
Neoschizomers: BstKT I | ↓GATC | |
| |
|
|
|
Product |
|
|
|
Cat. No. |
|
|
|
Amount |
|
|
|
Price (EUR) |
|
|
|
Buy |
|
|
|
|
|
|
|
S pack |
|
|
|
EN-150S |
|
|
|
500 Units |
|
|
|
25,00 |
|
|
|
 |
|
|
|
 |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
L pack |
|
|
|
EN-150L |
|
|
|
2500 Units |
|
|
|
100,00 |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
| | Source: Streptomyces species
Buffer supplied: 10x B2 (incl. 10x BSA).
Substrate for unit definition: λ DNA
Reaction conditions: 50 mM NaCl, 10 mM Tris-HCl (pH 7.9 @ 25°C), 10 mM MgCl2, 1 mM DTT, 100 µg/ml BSA. Incubate at 37°C. | | Storage buffer: 50 mM KCl, 10 mM Tris-HCl (pH 7.4), 0.1 mM EDTA, 1 mM dithiothreitol, 200 µg/ml BSA and 50 % glycerol. Store at -20°C.
Ligation and recutting: After ten-fold overdigestion with Sau3A I, >95 % of the DNA fragments can be ligated and recut with this enzyme.
Heat inactivation: 65°C for 20 minutes. | | |
|
