 | Source: An E. coli strain that carries the cloned Pst I gene from Providencia stuartii.
Buffer supplied: 10x Pst I (incl. 10x BSA).
Substrate for unit definition: λ DNA (28 sites).
Reaction conditions: 100 mM NaCl, 50 mM Tris-HCl (pH 7.4 @ 25°C), 10 mM MgCl2, 1 mM dithiothreitol, 100 µg/ml BSA. Incubate at 37°C.
Storage buffer: 200 mM NaCl, 10 mM Tris-HCl (pH 7.4), 0.1 mM EDTA, 1 mM dithiothreitol, 200 µg/ml BSA, 0.15 % Triton X-100 and 50 % glycerol. Store at -20°C. | | Ligation and recutting: After ten-fold overdigestion with Pst I, >95 % of the DNA fragments can be ligated and recut with this enzyme.
Star activity: Conditions of high enzyme concentration or glycerol concentration >12 % may result in star activity.
Heat inactivation: 80°C for 20 minutes. | |
