 | Source: Psychrobacter immobilis TA137.
Buffer supplied: 10x B2 (incl. 10x BSA).
Substrate for unit definition: λ DNA (74 sites).
Reaction conditions: 50 mM NaCl, 10 mM Tris-HCl (pH 7.9 @ 25°C), 10 mM MgCl2, 1 mM dithiothreitol, 100 µg/ml BSA. Incubate at 25°C.
Storage buffer: 50 mM NaCl, 10 mM Tris-HCl (pH 7.4 @ 25°C), 0.1 mM EDTA, 1 mM dithiothreitol, 200 µg/ml BSA and 50 % glycerol. Store at -20°C. | | Ligation and recutting: After ten-fold overdigestion with PspP I, >95 % of the DNA fragments can be ligated and recut with this enzyme.
Note: Incubation at 37°C results in 60 % activity. Blocked by overlapping dcm methylation.
Heat inactivation: 55°C for 15 minutes. | |
