Recognition Sequence:


Source: Nocardia rubra.

Buffer supplied: 10x Reation Buffer Nru I.

Storage buffer: 50 mM KCl, 10 mM Tris-HCl (pH 7.4), 0.1 mM EDTA,
1 mM dithiothreitol, 200 µg/ml BSA and 50 % [v/v] glycerol.
Store at -20°C.

Reaction conditions: 100 mM KCI, 50 mM Tris-HCl (pH 8.0 at 25°C), 10 mM MgCl₂ and 100 µg/ml BSA.
Incubate at 37°C.

Heat inactivation: 20 minutes at 65°C.

Ligation and recutting: After ten-fold overdigestion with Nru I,
<20 % of the DNA fragments can be ligated.

Star activity: Large excess of the enzyme results in the appearance of star activity.

DNA Methylation:
No Inhibition: dcm
Inhibition (Blocked): CpG
Inhibition (Blocked by overlapping): dam

Unit Definition: One unit is the amount of enzyme required to completely digest 1 µg of Lambda DNA (5 sites) in 1 hour in a total reaction volume of 50 µl. Enzyme activity was determined in the recommended reaction buffer.