 | Source: Nocardia rubra.
Buffer supplied: 10x Nru I and 10x BSA.
Substrate for unit definition: λ DNA (5 sites).
Reaction conditions: 100 mM KCI, 50 mM Tris-HCl (pH 8.0 @ 25°C), 10 mM MgCl2, 100 µg/ml BSA. Incubate at 37°C.
Storage buffer: 50 mM KCl, 10 mM Tris-HCl (pH 7.4), 0.1 mM EDTA, 1 mM dithiothreitol, 200 µg/ml BSA and 50 % glycerol. Store at -20°C. | | Ligation and recutting: After ten-fold overdigestion with Nru I, <20 % of the DNA fragments can be ligated.
Star activity: Large excess of the enzyme results in the appearance of star activity.
Heat inactivation: 65°C for 20 minutes.
Note: Blocked by overlapping dam methylation. | |
