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"M" Enzymes - MspC I| MspC I Isoschizomers: Afl II, BspT I, Bst98 I, Vha464 I |
C↓TTAAG | ||||
| Product | Cat. No. | Amount | Price (EUR) | Buy / Note | |
|---|---|---|---|---|---|
| S pack | EN-121S | 1300 Units | 25,00 | 
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| L pack | EN-121L | 6500 Units | 100,00 |
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Recognition Sequence:
Source: Micrococcus species.
Buffer supplied: 10x Reaction Buffer B4.
Storage buffer: 50 mM KCl, 10 mM Tris-HCl (pH 7.9), 0.1 mM EDTA,
1 mM dithiothreitol, 200 µg/ml BSA and 50 % [v/v] glycerol.
Store at -20°C.
Reaction conditions: 150 mM NaCl, 10 mM Tris-HCl (pH 7.9 at 25°C), 10 mM MgCl2, 1 mM dithiothreitol and 100 µg/ml BSA.
Incubate at 37°C.
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Source: Micrococcus species.
Buffer supplied: 10x Reaction Buffer B4.
Storage buffer: 50 mM KCl, 10 mM Tris-HCl (pH 7.9), 0.1 mM EDTA,
1 mM dithiothreitol, 200 µg/ml BSA and 50 % [v/v] glycerol.
Store at -20°C.
Reaction conditions: 150 mM NaCl, 10 mM Tris-HCl (pH 7.9 at 25°C), 10 mM MgCl2, 1 mM dithiothreitol and 100 µg/ml BSA.
Incubate at 37°C.
Heat inactivation: 65°C for 20 minutes.
Ligation and recutting: After 5-fold overdigestion with MspC I,
>95 % of the DNA fragments can be ligated and recut with this enzyme.
DNA Methylation: No Inhibition: dam, dcm, CpG
Unit Definition: One unit is the amount of enzyme required to completely digest 1 µg of Lambda DNA (Hind III digest; 3 sites) in 1 hour in a total reaction volume of 50 µl. Enzyme activity was determined in the recommended reaction buffer.
Ligation and recutting: After 5-fold overdigestion with MspC I,
>95 % of the DNA fragments can be ligated and recut with this enzyme.
DNA Methylation: No Inhibition: dam, dcm, CpG
Unit Definition: One unit is the amount of enzyme required to completely digest 1 µg of Lambda DNA (Hind III digest; 3 sites) in 1 hour in a total reaction volume of 50 µl. Enzyme activity was determined in the recommended reaction buffer.
Please contact enzymes@jenabioscience.com for more information.