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"M" Enzymes - Mbo I| Mbo I Isoschizomers: BfuC I, Bsp143 I, BstEN II, BstMB I, Dpn II, Kzo9 II, NdeI I Neoschizomers: BstKT I |
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| Product | Cat. No. | Amount | Price (EUR) | Buy / Note | |
|---|---|---|---|---|---|
| S pack | EN-120S | 300 Units | 25,00 | 
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| L pack | EN-120L | 1500 Units | 100,00 |
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Recognition Sequence:
Source: Moraxella bovis, recombinant, Streptomyces spezies.
Buffer supplied: 10x Reaction Buffer Mbo I.
Storage buffer: 50 mM KCl, 10 mM Tris-HCl (pH 7.4), 0.1 mM EDTA,
1 mM dithiothreitol, 200 µg/ml BSA and 50 % [v/v] glycerol.
Store at -20°C.
Reaction conditions: 100 mM KCl, 10 mM Tris-HCl (pH 8.0 at 25°C), 10 mM MgCl2, 1mM DTT and 100 µg/ml BSA.
Incubate at 37°C.
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Source: Moraxella bovis, recombinant, Streptomyces spezies.
Buffer supplied: 10x Reaction Buffer Mbo I.
Storage buffer: 50 mM KCl, 10 mM Tris-HCl (pH 7.4), 0.1 mM EDTA,
1 mM dithiothreitol, 200 µg/ml BSA and 50 % [v/v] glycerol.
Store at -20°C.
Reaction conditions: 100 mM KCl, 10 mM Tris-HCl (pH 8.0 at 25°C), 10 mM MgCl2, 1mM DTT and 100 µg/ml BSA.
Incubate at 37°C.
Heat inactivation: 20 minutes at 65°C.
Ligation and recutting: After ten-fold overdigestion with Mbo I,
>95 % of the DNA fragments can be ligated and recut with this enzyme.
DNA Methylation:
No Inhibition: dcm
Inhibition (Impaired by overlapping): CpG
Inhibition (Blocked): dam
Unit Definition: One unit is the amount of enzyme required to completely digest 1 µg of Lambda DNA (dam-; 116 sites) in 1 hour in a total reaction volume of 50 µl. Enzyme activity was determined in the recommended reaction buffer.
Ligation and recutting: After ten-fold overdigestion with Mbo I,
>95 % of the DNA fragments can be ligated and recut with this enzyme.
DNA Methylation:
No Inhibition: dcm
Inhibition (Impaired by overlapping): CpG
Inhibition (Blocked): dam
Unit Definition: One unit is the amount of enzyme required to completely digest 1 µg of Lambda DNA (dam-; 116 sites) in 1 hour in a total reaction volume of 50 µl. Enzyme activity was determined in the recommended reaction buffer.
Please contact enzymes@jenabioscience.com for more information.