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Mbo I
Isoschizomers: BfuC I, Bsp143 I, BstEN II, BstMB I, Dpn II, Kzo9 II, NdeI I
Neoschizomers: BstKT I | ↓GATC | |
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S pack |
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EN-120S |
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300 Units |
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25,00 |
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L pack |
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EN-120L |
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1500 Units |
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100,00 |
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| | Source: A Streptomyces sp. strain that carries the cloned Mbo I gene from Moraxella bovis.
Buffer supplied: 10x Mbo I and 10x BSA.
Substrate for unit definition: λ DNA dam- (116 sites).
Reaction conditions: 100 mM KCl, 10 mM Tris-HCl (pH 8.0 @ 25°C), 10 mM MgCl2, 1mM DTT, 100 µg/ml BSA. Incubate at 37°C. | | Storage buffer: 50 mM KCl, 10 mM Tris-HCl (pH 7.4), 0.1 mM EDTA, 1 mM dithiothreitol, 200 µg/ml BSA and 50 % glycerol. Store at -20°C.
Ligation and recutting: After ten-fold overdigestion with Mbo I, >95 % of the DNA fragments can be ligated and recut with this enzyme.
Heat inactivation: 65°C for 20 minutes.
Note: Blocked by overlapping dam methylation. | | |
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