 | Source: An E. coli strain that carries the cloned Hpa I gene from Haemophilus parainfluenzae.
Buffer supplied: 10x B5 (incl. 10x BSA).
Substrate for unit definition: λ DNA (14 sites).
Reaction conditions: 50 mM potassium acetate, 20 mM Tris-acetate (pH 7.9 @ 25°C), 10 mM magnesium acetate, 1 mM dithiothreitol, 100 µg/ml BSA. Incubate at 37°C. | | Storage buffer: 50 mM KCl, 10 mM Tris-HCl (pH 7.4), 0.1 mM EDTA, 1 mM dithiothreitol , 500 µg/ml BSA and 50 % glycerol. Store at -20°C.
Ligation and recutting: After ten-fold overdigestion with Hpa I, >95 % of the DNA fragments can be ligated and recut with this enzyme.
Star activity: Conditions of high enzyme concentration or glycerol concentration >5 %, may result in star activity.
Heat inactivation: No. | |
