 | Source: Haemophilus influenzae Rd.
Buffer supplied: 10x B2 (incl. 10x BSA).
Substrate for unit definition: λ DNA (7 sites).
Reaction conditions: 50 mM NaCl, 10 mM Tris-HCl (pH 7.9 @ 25°C), 10 mM MgCl2, 1 mM dithiothreitol, 100 µg/ml BSA. Incubate at 37°C. | | Storage buffer: 50 mM KCl, 10 mM Tris-HCl (pH 7.4), 0.1 mM EDTA, 1 mM dithiothreitol, 500 µg/ml BSA and 50 % glycerol. Store at -20°C.
Ligation and recutting: After 100-fold overdigestion with Hind III, >98 % of the DNA fragments can be ligated and recut with this enzyme.
Star activity: Star activity may be observed in the presence of Mn2+.
Heat inactivation: 65°C for 20 minutes. | |
