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"C" Enzymes - CspA I| CspA I Isoschizomers: Age I, AsiA I, BshT I, PinA I |
A↓CCGGT | ||||
| Product | Cat. No. | Amount | Price (EUR) | Buy / Note | |
|---|---|---|---|---|---|
| S pack | EN-113S | 150 Units | 25,00 | 
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| L pack | EN-113L | 750 Units | 100,00 |
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Recognition Sequence:
Source: Corynebacterium species.
Buffer supplied: 10x Reaction Buffer CspA I.
Storage buffer: 100 mM KCl, 10 mM Tris-HCl (pH 7.4), 0.1 mM EDTA,
1 mM dithiothreitol, 200 µg/ml BSA and 50 % [v/v] glycerol.
Store at -20°C.
Reaction conditions: 10 mM Bis Tris Propane-HCl (pH 7.0 at 25°C),
10 mM MgCl2, 1 mM dithiothreitol and 100 µg/ml BSA.
Incubate at 37°C.
5
'
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A
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C
C
G
G
T
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3
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3
'
-
T
G
G
C
C
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A
-
5
'
Source: Corynebacterium species.
Buffer supplied: 10x Reaction Buffer CspA I.
Storage buffer: 100 mM KCl, 10 mM Tris-HCl (pH 7.4), 0.1 mM EDTA,
1 mM dithiothreitol, 200 µg/ml BSA and 50 % [v/v] glycerol.
Store at -20°C.
Reaction conditions: 10 mM Bis Tris Propane-HCl (pH 7.0 at 25°C),
10 mM MgCl2, 1 mM dithiothreitol and 100 µg/ml BSA.
Incubate at 37°C.
Heat inactivation: 20 minutes at 65°C.
Ligation and recutting: After ten-fold overdigestion with CspA I,
>90 % of the DNA fragments can be ligated and recut with this enzyme.
DNA Methylation: No Inhibition: dam, dcm, CpG
Unit Definition: One unit is the amount of enzyme required to completely digest 1 µg of Lambda DNA (13 sites) in 1 hour in a total reaction volume of 50 µl. Enzyme activity was determined in the recommended reaction buffer.
Ligation and recutting: After ten-fold overdigestion with CspA I,
>90 % of the DNA fragments can be ligated and recut with this enzyme.
DNA Methylation: No Inhibition: dam, dcm, CpG
Unit Definition: One unit is the amount of enzyme required to completely digest 1 µg of Lambda DNA (13 sites) in 1 hour in a total reaction volume of 50 µl. Enzyme activity was determined in the recommended reaction buffer.
Please contact enzymes@jenabioscience.com for more information.