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CspA I
Isoschizomers: Age I, AsiA I, BshT I, PinA I | A↓CCGGT | |
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Cat. No. |
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Price (EUR) |
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S pack |
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EN-113S |
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150 Units |
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25,00 |
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L pack |
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EN-113L |
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750 Units |
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100,00 |
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| | Source: Corynebacterium species.
Buffer supplied: 10x CspA I and 10x BSA.
Substrate for unit definition: λ DNA (13 sites).
Reaction conditions: 10 mM Bis Tris Propane-HCl (pH 7.0 @ 25°C), 10 mM MgCl2, 1 mM dithiothreitol, 100 µg/ml BSA. Incubate at 37 °C. | | Storage buffer: 100 mM KCl, 10 mM Tris-HCl (pH 7.4 @ 25°C), 0.1 mM EDTA, 1 mM dithiothreitol, 200 µg/ml BSA and 50 % glycerol. Store at -20°C.
Ligation and recutting: After ten-fold overdigestion with CspA I, >90 % of the DNA fragments can be ligated and recut with this enzyme.
Heat inactivation: 65°C for 20 minutes.
Note: Blocked by overlapping dcm methylation. | | |
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