Recognition Sequence:


Source: Bacillus stearothermophilus.

Buffer supplied: 10x Reaction Buffer BstE II.

Storage buffer: 100 mM KCl, 10 mM Tris-HCl (pH 7.4), 0.1 mM EDTA,
1 mM dithiothreitol, 200 µg/ml BSA and 50 % [v/v] glycerol.
Store at -20°C.

Reaction conditions: 100 mM KCl, 10 mM Tris-HCl (pH 7.4 at 25°C),
5 mM MgCl₂, 1 mM dithiothreitol, 0.1 % Triton X-100 and 100 µg/ml BSA.
Incubate at 60°C.

Heat inactivation: No.

Ligation and recutting: After forty-fold overdigestion with BstE II, >95 % of the DNA fragments can be ligated and recut with this enzyme.

Note: BstE II exhibits 10 - 15 % activity at 37°C.

DNA Methylation: No Inhibition: dam, dcm, CpG

Unit Definition: One unit is the amount of enzyme required to completely digest 1 µg of Lambda DNA (13 sites) in 1 hour in a total reaction volume of 50 µl. Enzyme activity was determined in the recommended reaction buffer.