 | Source: Bacillus stearothermophilus.
Buffer supplied: 10x BstE II and 10x BSA.
Substrate for unit definition: λ DNA (13 sites).
Reaction conditions: 100 mM KCl, 10 mM Tris-HCl (pH 7.4 @ 25°C), 5 mM MgCl2, 1 mM dithiothreitol, 0.1 % Triton X-100, 100 µg/ml BSA. Incubate at 60°C. | | Storage buffer: 100 mM KCl, 10 mM Tris-HCl (pH 7.4), 0.1 mM EDTA, 1 mM dithiothreitol, 200 µg/ml BSA and 50 % glycerol. Store at -20°C.
Ligation and recutting: After forty-fold overdigestion with BstE II, >95 % of the DNA fragments can be ligated and recut with this enzyme.
Note: BstE II exhibits 10 - 15 % activity at 37°C.
Heat inactivation: No. | |
