 | Source: Bacillus species.
Buffer supplied: 10x BssA I and 10x BSA.
Substrate for unit definition: λ DNA (61 sites).
Reaction conditions: 100 mM KCl, 20 mM Tris-HCl (pH 8.5 @ 25°C), 3 mM MgCl2, 0.04 % Triton X-100, 100 µg/ml BSA. Incubate at 65°C. | | Storage buffer: 50 mM KCl, 10 mM Tris-HCl (pH 7.4), 0.1 mM EDTA, 1 mM dithiothreitol, 200 µg/ml BSA and 50 % glycerol. Store at -20°C.
Ligation and recutting: After 30-fold overdigestion with BssA I, >95 % of the DNA fragments can be ligated and recut with this enzyme.
Star activity: Low salt concentration or large excess of enzyme results in the appearance of star activity.
Heat inactivation: No. | |
