|
|
 |
|
| |
BshF I
Isoschizomers: BspAN I, BsuR I, Hae III, Pal I, Pho I | GG↓CC | |
| |
|
|
|
Product |
|
|
|
Cat. No. |
|
|
|
Amount |
|
|
|
Price (EUR) |
|
|
|
Buy |
|
|
|
|
|
|
|
S pack |
|
|
|
EN-110S |
|
|
|
7000 Units |
|
|
|
25,00 |
|
|
|
 |
|
|
|
 |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
L pack |
|
|
|
EN-110L |
|
|
|
35000 Units |
|
|
|
100,00 |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
| | Source: Bacillus sphaericus.
Buffer supplied: 10x B5 (incl. 10x BSA).
Substrate for unit definition: λ DNA (149 sites).
Reaction conditions: 50 mM potassium acetate, 20 mM Tris-acetate (pH 7.9 @ 25°C), 10 mM magnesium acetate, 1 mM dithiothreitol, 100 µg/ml BSA. Incubate at 37°C. | | Storage buffer: 50 mM KCl, 10 mM Tris-HCl (pH 7.4), 0.1 mM EDTA, 1 mM dithiothreitol, 200 µg/ml BSA and 50 % glycerol. Store at -20°C.
Ligation and recutting: After ten-fold overdigestion with BshF I, >95 % of the DNA fragments can be ligated and recut with this enzyme.
Heat inactivation: 80°C for 20 minutes.
Note: Blocked by overlapping dcm methylation. | | |
|
