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BseC I
Isoschizomers: Ban III, Bsa29 I, Bsp106 I, BspD I, BspX I, Bsu15 I, BsuTU I, Cla I, Zho I | AT↓CGAT | |
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Cat. No. |
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Amount |
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Price (EUR) |
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Buy |
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S pack |
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EN-109S |
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3500 Units |
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25,00 |
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L pack |
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EN-109L |
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17500 Units |
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100,00 |
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| | Source: Bacillus stearothermophilus.
Buffer supplied: 10x B3 (incl. 10x BSA).
Substrate for unit definition: λ DNA (15 sites).
Reaction conditions: 100 mM NaCl, 50 mM Tris-HCl (pH 7.9 @ 25°C), 10 mM MgCl2, 1 mM dithiothreitol, 100 µg/ml BSA. Incubate at 55°C. | | Storage buffer: 100 mM KCl, 10 mM Tris-HCl (pH 7.9 @ 25°C), 0.1 mM EDTA, 1 mM dithiothreitol, 0.15 % Triton X-100, 200 µg/ml BSA and 50 % glycerol. Store at -20°C.
Ligation and recutting: After 30-fold overdigestion with BseC I, >95 % of the DNA fragments can be ligated and recut with this enzyme.
Heat inactivation: No.
Note: Blocked by overlapping dam methylation. | | |
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