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BseA I
Isoschizomers: Acc III, Bsp13 I, BspE I, Kpn2 I, Mro I | T↓CCGGA | |
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S pack |
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EN-107S |
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650 Units |
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25,00 |
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L pack |
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EN-107L |
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3250 Units |
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100,00 |
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| | Source: Bacillus stearothermophilus.
Buffer supplied: 10x BseA I and 10x BSA.
Substrate for unit definition: λ DNA (24 sites).
Reaction conditions: 100 mM NaCl, 10 mM Tris-HCl (pH 8.0 @ 25°C), 5 mM MgCl2, 1 mM dithiothreitol, 0.02 % Triton X-100, 100 µg/ml BSA. Incubate at 55°C. | | Storage buffer: 50 mM KCl, 10 mM Tris-HCl (pH 7.4), 0.1 mM EDTA, 1 mM dithiothreitol, 500 µg/ml BSA and 50 % glycerol. Store at -20°C.
Ligation and recutting: After 40-fold overdigestion with BseA I, >98 % of the DNA fragments can be ligated and recut with this enzyme.
Heat inactivation: No.
Note : Impaired by overlapping dcm methylation. Blocked by overlapping dam methylation. | | |
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