Recognition Sequence:


Source: Bacillus globigii lacking Bgl II.

Buffer supplied: 10x Reaction Buffer Bgl I.

Storage buffer: 200 mM NaCl, 20 mM Tris-HCl (pH 7.5), 0.1 mM EDTA, 1 mM dithiothreitol, 200 µg/ml BSA and 50 % [v/v] glycerol.
Store at -20°C.

Reaction conditions: 50 mM NaCl, 100 mM Tris-HCl (pH 7.9 at 25°C), 5 mM MgCl₂, 0.025 % Triton X-100 and 100 µg/ml BSA.
Incubate at 37°C.

Heat inactivation: 20 minutes at 65°C.

Ligation and recutting: After ten-fold overdigestion with Bgl I, >95 % of the DNA fragments can be ligated and recut with this enzyme.

DNA Methylation:
No inhibition: dam, dcm
Inhibition (Blocked by some combinations of overlapping): CpG

Unit Definition: One unit is the amount of enzyme required to completely digest 1 µg of Lambda DNA (29 sites) in 1 hour in a total reaction volume of 50 µl. Enzyme activity was determined in the recommended reaction buffer.