 | Source: Bacillus globigii lacking Bgl II.
Buffer supplied: 10x Bgl I and 10x BSA.
Substrate for unit definition: λ DNA (29 sites).
Reaction conditions: 50 mM NaCl, 100 mM Tris-HCl (pH 7.9 @ 25°C), 5 mM MgCl2, 0.025 % Triton X-100, 100 µg/ml BSA. Incubate at 37°C. | | Storage buffer: 200 mM NaCl, 20 mM Tris-HCl (pH 7.5), 0.1 mM EDTA, 1 mM dithiothreitol, 200 µg/ml BSA and 50 % glycerol. Store at -20°C.
Ligation and recutting: After ten-fold overdigestion with Bgl I, >95 % of the DNA fragments can be ligated and recut with this enzyme.
Heat inactivation: 65°C for 20 minutes. | |
