 | Source: Bacillus caldolyticus.
Buffer supplied: 10x B2 (incl. 10x BSA).
Substrate for unit definition: λ DNA dam- (8 sites).
Reaction conditions: 50 mM NaCl, 10 mM Tris-HCl (pH 7.9 @ 25°C), 10 mM MgCl2, 1 mM dithiothreitol, 100 µg/ml BSA. Incubate at 50°C.
Storage buffer: 50 mM KCl, 10 mM Tris-HCl (pH 7.4), 0.1 mM EDTA, 1 mM dithiothreitol, 200 µg/ml BSA, and 50 % glycerol. Store at -20°C. | | Ligation and recutting: After ten-fold overdigestion with Bcl I, >95 % of the DNA fragments can be ligated and recut with this enzyme.
Star activity: Large excess of the enzyme results in the appearance of star activity.
Heat inactivation: No.
Note: Blocked by dam methylation. | |
