Recognition Sequence:


Source: Bacillus amyloliquefaciens H.

Buffer supplied: 10x Reaction Buffer BamH I

Storage buffer: 50 mM KCl, 10 mM Tris-HCl (pH 7.4), 0.1 mM EDTA, 1 mM dithiothreitol, 200 µg/ml BSA and 50 % [v/v] glycerol.
Store at -20°C.

Reaction conditions: 100 mM NaCl, 10 mM Tris-HCl (pH 7.9 at 25°C), 5 mM MgCl₂, 1 mM dithiothreitol and 100 µg/ml BSA.
Incubate at 37°C.

Heat inactivation: 20 minutes at 80°C.

Ligation and recutting: After 50-fold overdigestion with BamH I,
>95 % of the DNA fragments can be ligated and recut with this enzyme.

Star activity: Conditions of low ionic strength, high enzyme concentration, glycerol concentration >5 %, or pH >8.0 may result in star activity.

DNA Methylation: No Inhibition: dam, dcm, CpG

Unit Definition: One unit is the amount of enzyme required to completely digest 1 µg of Lambda DNA (5 sites) in 1 hour in a total reaction volume of 50 µl. Enzyme activity was determined in the recommended reaction buffer.