 | Source: Bacillus amyloliquefaciens H.
Buffer supplied: 10x BamH I (incl. 10x BSA).
Substrate for unit definition: λ DNA (5 sites).
Reaction conditions: 100 mM NaCl, 10 mM Tris-HCl (pH 7.9 @ 25°C), 5 mM MgCl2, 1 mM dithiothreitol, 100 µg/ml BSA. Incubate at 37°C.
Storage buffer: 50 mM KCl, 10 mM Tris-HCl (pH 7.4), 0.1 mM EDTA, 1 mM dithiothreitol, 200 µg/ml BSA and 50 % glycerol. Store at -20°C. | | Ligation and recutting: After 50-fold overdigestion with BamH I, >95 % of the DNA fragments can be ligated and recut with this enzyme.
Star activity: Conditions of low ionic strength, high enzyme concentration, glycerol concentration >5 %, or pH >8.0 may result in star activity.
Heat inactivation: 80°C for 20 minutes. | |
