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  Real-Time PCR - qPCR Supplements
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qPCR Mixes for Dual Labeled Fluorescent Probes   |   qPCR Mixes with EvaGreen   |   qPCR Core Kits   |   qPCR Supplements   |   Dual Labeled Fluorescent Probes
 

  
qPCR Supplements

Helpful tools for Real-Time PCR, to test the function or control the quality of qPCR polymerases and qPCR assay conditons.


 Fluorescein Reference Dye 
Product Cat. No. Amount Price (EUR) Buy  
PCR-355 1,000 reactions 25,00  
Store at -20°C in the dark

100x FAM reference dye, 500 µl
FAM reference dye is optimized for block-based real-time PCR instruments that are compatible with the evaluation of the FAM reference signal. The dye does not take part in the PCR reaction but allows to normalize for non-PCR related signal variation and provides a baseline in multiplex reactions. The dye is supplied as 100x concentration.
 ROX Reference Dye 
Product Cat. No. Amount Price (EUR) Buy  
PCR-351 500 reactions 25,00  
Store at -20°C in the dark

50x ROX reference dye, 500 µl
ROX reference dye is optimized for block-based real-time PCR instruments that are compatible with the evaluation of the ROX reference signal. The dye does not take part in the PCR reaction but allows to normalize for non-PCR related signal variation and provides a baseline in multiplex reactions. The dye is supplied as 50x concentration.
 EvaGreenTM Fluorescent DNA Stain 
Product Cat. No. Amount Price (EUR) Buy  
PCR-352 500 reactions 40,00  
Store at -20°C in the dark

50x EvaGreenTM Fluorescent DNA Stain, 500 µl
EvaGreenTM Fluorescent DNA Stain is a superior DNA intercalator dye specially developed for DNA analysis applications including real-time PCR (qPCR) and high-resolution DNA melt curve analysis (HRM). Upon binding to DNA, the non-fluorescent dye becomes highly fluorescent while showing no detectable inhibition to the PCR process. The dye is extremely stable both thermally and hydrolytically, providing convenience during routine handling.
The high quantum yield, excellent stability and less inhibition toward PCR makes it the ideal fluorophore in real-time PCR applications as replacement for the widely used SYBR® Green I dye.
 Thermolabile UNG (Uracil N-Glycosylase) 
Product Cat. No. Amount Price (EUR) Buy  
PCR-353 200 units 100,00  
Store at -20°C

Uracil N-Glycosylase, 200 units (1 unit/µl)
Uracyl N-Glycosylase (UNG, UDG) catalyses the release of uracil from single and double stranded uracyl-containing DNA. The resulting abasic sites are susceptible to hydrolytic cleavage at elevated temperatures.
UNG is used in real-time PCR to prevent carry-over contaminations of dU-containing DNA from previous reactions.
 qPCR Control Kit 
Product Cat. No. Amount Price (EUR) Buy  
PCR-354 500 reactions 130,00  
Store at -20°C
Dual labeled fluorescent probe must be stored in the dark

ß-actin forward primer
ß-actin reverse primer
Dual labeled fluorescent probe, FAM-TAMRA
Human genomic DNA (male)
PCR Control Kit is designed as tool to confirm or compare the quality and functionality of quantitative real-time analysis using DNA probe based detection. The kit contains a forward / reverse primer-pair and the dual labeled fluorescent probe designed to detect the β-actin gene from human genomic DNA.
 dNTP Mix GCamplifier
 Modified dNTP Mix for amplification of GC-rich sequences
 
Product Cat. No. Amount Price (EUR) Buy  
Kit PCR-257 100 µl 80,00  
Store at -20°C

10 mM each dNTP
dNTP PCR Mix GCamplifier is specially designed for amplification of GC-rich DNA templates. The kit contains a modified dGTP analog that significantly reduces the stability of GC-rich sequences thereby enhancing PCR efficiency. The dNTP PCR Mix GCamplifier is used instead of a standard dNTP mix.



EvaGreen® is a registered trademark and licensed for sale by Biotium, Inc., Hayward, CA, USA.
SYBR® is a registered trademark of Invitrogen Corporation, Carlsbad, California, USA.


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