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Emerald BioSystems Crystal Growth Matrices - Precipitant Synergy™ |
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Precipitant Synergy™
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Precipitant Synergy™ uses synergistic combinations of mechanistically distinct precipitating agents to obtain crystallization hits from a starter set of 64 different formulations. Each Precipitant Synergy™ (PS) formulation contains a carefully selected combination of at least two mechanistically distinct precipitating agents including:
- salts that affect the activity coefficient of water at high concentration
- organic solvents which alter the dielectric constant of the solvating medium
- PEGs which operate by increasing molecular crowding
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Tests with 10 different proteins showed that the Precipitant Synergy™ screen roughly triples the number of different crystals obtained compared to other screens [1].
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Each of the 64 formulations in the Primary PS screen are prepared with precipitants at a fairly high concentration, close to their maximum solubility, in order to reach or exceed the protein’s precipitation point. The 64 PS formulations also encompass a broad pH range from 5.5 to 8.5, with a diverse array of eight different buffering agents.
Rapid optimization of initial hits (precipitation and/or crystallization) is achieved by either serial dilution of the lead Primary PS formulation, or by re-screening with Expanded PS formulations that contain precipitating agents at 67% or 33% of their primary concentration, while holding the buffer and additive compositions constant. Hence, the 192-formulaion Expanded format has three sets of the 64 PS formulations with precipitant concentrations at 100% (Primary 64), 67%, or 33%.
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Reference:
[1] Majeed et al. (2003) Enhancing Protein Crystallization through Precipitant Synergy. Structure 11:1061.
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Individual Conditions of all screens are available in 200 ml as well as 500 ml volumes. Please follow this link.
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Nonsaleable to USA and Japan! Please contact Emerald BioSystems for distributor information.
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Downloads |
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Precipitant Synergy™ Primary 64
64 formulations, 10 ml each |
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EBS-TPS-P1 |
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1 Kit |
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525,00 |
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Precipitant Synergy™ Primary 64 in 96 well block
64 formulations, 1.7 ml each |
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EBS-BPS-P1 |
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1 Block |
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300,00 |
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Precipitant Synergy™ Expanded 192
192 formulations, 10 ml each |
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EBS-TPS-E |
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1 Kit |
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1.400,00 |
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Precipitant Synergy™ Expanded 192 in 96 well block
192 formulations, 1.7 ml each |
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EBS-BPS-E |
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3 Blocks |
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800,00 |
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