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Applications for Modified Nucleotides - DNA Manipulation |
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| Random Mutagenesis | | |
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8-Oxo-dGTP, S pack |
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NU-1117S |
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30 Units |
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95,00 |
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8-Oxo-dGTP, L pack |
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NU-1117L |
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150 Units |
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300,00 |
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dPTP, S pack |
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NU-1119S |
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30 Units |
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95,00 |
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dPTP, L pack |
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NU-1119L |
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150 Units |
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300,00 |
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Description
Modified nucleotides for random mutagenesis including
lethal or HIV mutagenesis
| Incorporation
Taq DNA Polymerase
Reverse Transcriptase (8-Oxo-dGTP)
References
Zaccolo et al. (1996) An Approach to Random Mutagenesis of DNA Using Mixtures of Triphosphate Derivatives of Nucleoside Analogues. Journal of Molecular Biology 255:589.
Lethal mutagenesis:
Loeb et al. (1999) Lethal mutagenesis of HIV with mutagenic nucleoside analogs. Proc. Natl. Acad. Sci. USA 96:1492. |
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| Inactivation of Restriction Enzyme Recognition Sites | | |
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5-Methyl-dCTP, S pack |
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NU-1125S |
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100 Units |
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95,00 |
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5-Methyl-dCTP, L pack |
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NU-1125L |
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500 Units |
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300,00 |
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5-AcOHg-dCTP, S pack |
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NU-1149S |
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100 Units |
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95,00 |
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5-AcOHg-dCTP, L pack |
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NU-1149L |
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500 Units |
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300,00 |
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Description
5-Methyl-dCTP is widely used for construction of cDNA libraries
| Incorporation of 5-Methyl-dCTP
M-MuLV Reverse Transcriptase
Klenow Fragment of DNA Polymerase I
Sequenase DNA Polymerase
(Taq Polymerase, Vent) *
Incorporation of Hg-dCTP
DNA Polymerase I
References to 5-Methyl-dCTP
Lefaucheur et al. (1998) Evidence for three adult fast myosin heavy chain isoforms in type II skeletal muscle fibers in pigs. J. Anim. Sci. 76:1584.
Nelson et al. (1993) Restriction endonuclease cleavage of 5-methyl-deoxycytosine hemimethylated DNA at high enzyme-to-substrate ratios. Nucl. Acids Res. 21 (3):681.
Asamizu et al. (1999) A large scale structural analysis of cDNAs in a unicellular green alga, Chlamydomonas reinhardtii. I. Generation of 3433 non-redundant expressed sequence tags. DNA Research 6:369.
* Wong et al. (1991) PCR with 5-methyl-dCTP replacing dCTP. Nucl. Acids Res. 19 (5):1081.
Reference to Hg-dCTP
Banfalvi et al. (1995) Effect of mercury substitution of DNA on its susceptibility to cleavage by restriction endonucleases. DNA Cell Biol. 14 (5):445.
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| Inhibition of DNA Methylation | | |
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5-Aza-dCTP, S pack
(Decitabine triphosphate) |
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NU-1118S |
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20 Units |
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95,00 |
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5-Aza-dCTP, L pack
(Decitabine triphosphate) |
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NU-1118L |
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100 Units |
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300,00 |
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Description
5-Aza-dCTP is an inhibitor of DNA methylation and antileukemic agent. It has been used to reactivate silent tumor suppressor
genes.
| Incorporation
DNA Polymerase alpha
References
Bouchard et al. (1983) Incorporation of 5-Aza-2'-deoxycytidine-5'-triphosphate into DNA. Interactions with mammalian DNA polymerase alpha and DNA methylase. Molecular Pharmacology 24 (1):109.
Lemaire et al. (2005) Enhancement of antineoplastic action of 5-aza-2'-deoxycytidine by zebularine on L1210 leukemia. Anti-Cancer Drugs 16 (3):301. |
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| Selective RNA Amplification | | |
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dITP, solution
100 mM |
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NU-1007 |
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1 ml (100 µmol) |
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72,00 |
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Description
The nucleotide allows a selective RNA amplification in the
presence of genomic DNA by shifting the strand separation temperature
| Incorporation
Tth Polymerase
Reference
Auer et al. (1996) Selective amplification of RNA utilizing the nucleotide analog dITP and Thermus thermophilus DNA polymerase. Nucl. Acids Res. 24 (24):5021. |
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| Prevention of Transcription Arrests | | |
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5Br-dUTP, S pack |
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NU-122S |
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50 Units |
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95,00 |
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5Br-dUTP, L pack |
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NU-122L |
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250 Units |
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300,00 |
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Description
Modified nucleotides for higher efficiency of readthrough and prevention of transcription arrests
| Incorporation
RNA Polymerase II
E. coli RNA Polymerase
References
Mote et al. (1998) Recognition of a Human Arrest Site Is Conserved between RNA Polymerase II and Prokaryotic RNA Polymerases. J. Biological Chemistry 273 (27):16843.
Pal et al. (2002) Strong Natural Pausing by RNA Polymerase II within 10 Bases of Transcription Start May Result in Repeated Slippage and Reextension of the Nascent RNA. Molecular and Cellular Biology 22 (1):30. |
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For inquiries or further information, please contact us at pcr@jenabioscience.com
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