Catalog Chemical Environment - JBScreen DetergentsChemical Environment - JBScreen Detergents
 
 
JBScreen Detergents
JBScreen Detergents can be used throughout the protein purification process or can be added afterwards by dialysis or ion-exchange chromatography (detergent exchange). Detergent exchange can be vital for obtaining well-diffracting membrane-protein crystals [1].
JBScreen Detergents is also valuable for additive screening with detergents and detergent mixtures [2,3] in combination with the JBScreen Membrane. This combination will enable you to screen a broad range of crystallization conditions, while concentrating on the most successful detergents - and therefore making crystallization screening of membrane proteins much more efficient and less time consuming.

24 detergents are supplied as stock solutions at 5 or 10 times the reported CMC (Critical Micellar Concentration), with 100 or 200 Ál per compound. The recommended final detergent concentration in a hanging-drop experiment (prior to equilibration) is 1 to 3 x the CMC.
 

Product Cat. No. Amount Price (EUR) Buy / Note Downloads
JBScreen Detergents CD-103 24 solutions
(100 or 200 Ál each)
175,00    Add this product to your notepad  

References

[1] Rosenow et al. (2003) The influence of detergents and amphiphiles on the solubility of the light harvesting complex. Acta Cryst. D59:1422
[2] Adir (1999) Crystallization of the oxygen-evolving reaction centre of photosystem II in nine different detergent mixtures. Acta Cryst. D55:891
[3] Koronakis et al. (2000) Crystal structure of the bacterial membrane protein TolC central to multidrug efflux and protein export. Nature 405:914
 


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