Store at -20°C, avoid frequent thawing and freezing

Terminator A
Terminator C
Terminator G
Terminator T
Cycle Sequencing Polymerase
Cycle Sequencing Buffer
Stop Solution
PCR grade water

DNA Cycle Sequencing Kit is designed for DNA sequencing based on the Sanger Method (dideoxy chain termination method). It provides a powerful tool to derive rapidly DNA and gene sequence information as required in a multitude of molecular biological and biotechnological applications.
The performance of the kit is based on a modified Taq polymerase showing an equal capability of incorporating ddNTPs and dNTPs. This guarantees the generation of uniform and easy to read sequence band patterns at lowest background. A minimal band compression of GC-rich DNA regions is achieved by optimally balanced termination mixtures containing 7-deaza-dGTP.
The reaction chemistry of the kit is optimized for automated DNA sequencers and requires fluorescent-labeled primers.