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Phosphatases - Phosphatase Assay Kit |
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Protein phosphatases regulate fundamental biological processes such as cell proliferation and differentiation in cooperation with protein kinases. Protein phosphatases consist of three general families with different substrate specificities towards phospho-seryl/threonyl, -tyrosyl residues, or both.
This kit provides a simple means to measure phosphatase activity by using pNPP as a general substrate of phosphatases.
In a first step, the enzyme dephosphorylates its substrate pNPP. In a second step, the phenolic OH-group is deprotonated under alkaline conditions resulting in p-nitrophenolate with a strong absorption at 405 nm (yellow) (see figure on the right).
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Diagram on the left: Phosphatase SHP-1 (100 ng) was assayed with 10 mM pNPP in JBS Phosphatase Assay. This type of assay is called “end-point” assay since the activity of the enzyme is evaluated after a given time of reaction (here: 5, 10, 30 and 60 min).
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Phosphatase Assay Kit
pNPP-based colorimetric assay, 96 well format |
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PR-944 |
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1 Kit |
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180,00 |
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