(Picolyl-) Azides of fluorescent dyes can be used for the fluorescent labeling of terminal Alkyne and strained Alknye (e.g DBCO)-labeled molecules via Cu(I)-catalyzed Alknye-Azide (CUAAC) or Cu(I)-free strain-promoted Alkyne-Azide Click Chemistry (SPAAC) reaction, respectively.
Standard Dyes such as Cy3, Tamra or Texas Red have been thoroughly selected to cover the whole UV-Vis spectrum. Novel Alternative Dyes are available as well.
Emission color | Dye | Azide |
---|---|---|
blue | 3-Azido-7-hydroxycoumarin | 3-Azido-7-hydroxycoumarin |
green | BDP-FL (also known as BODIPY® FL) |
Azide - BDP-FL |
5-Fluorescein (5-FAM) | 5-FAM-Azide | |
6-Fluorescein (6-FAM) | 6-FAM-Azide | |
5/6-Fluorescein (5/6-FAM) | Picolyl-Azide-5/6-FAM | |
AF488 (also known as Alexa Fluor®488) |
AF488-Azide AF488-Picolyl-Azide |
|
5/6-Carboxyrhodamine 110 | 5/6-Carboxyrhodamine 110-PEG3-Azide | |
yellow | 5-TAMRA | 5-TAMRA-Azide |
5/6-TAMRA | 5/6-TAMRA-PEG3-Azide |
|
Cy3 | Cy3-Azide | |
Sulfo-Cy3 | Sulfo-Cy3-Azide Picolyl-Azide-Sulfo-Cy3 |
|
AF546 (also known as Alexa Fluor®546) |
AF546-Azide AF546-Picolyl-Azide |
|
AF555 (structural analog to Alexa Fluor®555) |
AF555-Azide AF555-Picolyl-Azide |
|
orange | AF594 (also known as Alexa Fluor®594) |
AF594-Azide AF594-Picolyl-Azide |
red | Cy5 | Cy5-Azide |
Sulfo-Cy5 | Sulfo-Cy5-Azide Picolyl-Azide-Sulfo-Cy5 |
|
AF647 (also known as Alexa Fluor®647) |
AF647-Azide AF647-Picolyl-Azide |
|
Cy5.5 | Cy5.5-Azide | |
near-IR | Cy7 | Picolyl-Azide-Cy7 |