Catalog LEXSY Configurations - Vectors and PrimersLEXSY Configurations - Vectors and Primers
 
 
Inducible (Genome integrated)   |   Inducible (Episomal)   |   Constitutive (Genome integrated)   |   Vectors and Primers   |   In Vitro (cell-free)   |   Custom Expression
 

The constitutive pLEXSY-2 vectors (EGE-231 to EGE-234) and inducible pLEXSY_I vectors (EGE-243 to EGE-251) were developed for both, intracellular or secretory expression of target proteins.


For constitutive expression we recommend the standard vector pLEXSY-sat2 for selection of LEXSY expression strains with the antibiotic Nourseothricin (NTC). For inducible expression we recommend the integrative vector pLEXSY_I-blecherry3 for selection of LEXSY expression strains with the antibiotic LEXSY Bleo and Cherry fluorescence monitoring of induction.

 

For expression plasmid construction we recommend our ligase-free CyClone technology in combination with our Primer Designer software (web shop account required)!

 

LEXSY Expression Vectors   
Product Cat. No. Amount Price (EUR) Buy / Note Downloads
pLEXSY-ble2
integrative constitutive expression vector
antibiotic selection of transfectants with LEXSY Bleo
EGE-231 5 g 480,00    Add this product to your notepad    
pLEXSY-hyg2
integrative constitutive expression vector
antibiotic selection of transfectants with LEXSY Hygro
EGE-232 5 g 480,00    Add this product to your notepad    
pLEXSY-neo2
integrative constitutive expression vector
antibiotic selection of transfectants with LEXSY Neo
EGE-233 5 g 480,00    Add this product to your notepad    
pLEXSY-sat2
integrative constitutive expression vector
antibiotic selection of transfectants with Nourseothricin (NTC)
EGE-234 5 g 480,00    Add this product to your notepad    
pLEXSY_I-blecherry3
integrative inducible expression vector
antibiotic selection of transfectants with LEXSY Bleo and expression monitoring with Cherry fluorescence
EGE-243 5 g 480,00    Add this product to your notepad    
pLEXSY_I-ble3
integrative inducible expression vector
antibiotic selection of transfectants with LEXSY Bleo
EGE-244 5 g 480,00    Add this product to your notepad    
pLEXSY_I-neo3
integrative inducible expression vector
antibiotic selection of transfectants with LEXSY Neo
EGE-245 5 g 480,00    Add this product to your notepad    
pLEXSY_IE-blecherry4
episomal inducible expression vector
antibiotic selection of transfectants with LEXSY Bleo and expression monitoring with Cherry fluorescence
EGE-255 5 g 480,00    Add this product to your notepad    

The LEXSY Control Vectors were developed as a positive transfection control. They allow evaluation of expression by simple fluorescence measurement of suspension cultures. As reporter proteins the Green, Red, or Cherry Fluorescence Proteins are available.

 

LEXSY Control Vectors   
Product Cat. No. Amount Price (EUR) Buy / Note Downloads
pLEXSY-egfp-sat2
integrative constitutive control vector with egfp gene
antibiotic selection of transfectants with Nourseothricin (NTC) and expression monitoring with EGFP fluorescence
EGE-235 5 g 480,00    Add this product to your notepad    
pLEXSY-cherry-sat2
integrative constitutive control vector with cherry gene
antibiotic selection of transfectants with Nourseothricin (NTC) and expression monitoring with Cherry fluorescence
EGE-236 5 g 480,00    Add this product to your notepad    
pLEXSY-red-sat2
integrative constitutive control vector with Ds gene
antibiotic selection of transfectants with Nourseothricin (NTC) and expression monitoring with Ds red fluorescence
EGE-237 5 g 480,00    Add this product to your notepad    
pLEXSY_I-egfp-blecherry3
integrative inducible control vector with egfp gene
antibiotic selection of transfectants with LEXSY Bleo and expression monitoring with EGFP and Cherry fluorescence
EGE-246 5 g 480,00    Add this product to your notepad    
pLEXSY_I-egfp-ble3
integrative inducible control vector with egfp gene
antibiotic selection of transfectants with LEXSY Bleo and expression monitoring with EGFP fluorescence
EGE-247 5 g 480,00    Add this product to your notepad    
pLEXSY_I-egfp-neo3
integrative inducible control vector with egfp gene
antibiotic selection of transfectants with LEXSY Neo and expression monitoring with EGFP fluorescence
EGE-248 5 g 480,00    Add this product to your notepad    

The LEXSY Primers were designed for insert sequencing and diagnostic PCR of recombinant LEXSY strains based on the LEXSY expression vectors.
 

Primers   
Product Cat. No. Amount Price (EUR) Buy / Note Downloads
A264
insert sequencing reverse primer
(for all expression vectors)
PM-101 2,5 nmol
(50 l)
22,00    Add this product to your notepad  
A992
insert sequencing forward primer
(for all 'F4' expression vectors with 5'utr camBA)
PM-102 2,5 nmol
(50 l)
22,00    Add this product to your notepad  
F2999
sat forward primer
(for integration diagnostics of all sat expression vectors)
PM-103 2,5 nmol
(50 l)
22,00    Add this product to your notepad  
F3002
ssu reverse primer
(for integration diagnostics of all ssu integration vectors)
PM-104 2,5 nmol
(50 l)
22,00    Add this product to your notepad  
F3001
ssu forward primer
(for integration diagnostics of all ssu integration vectors)
PM-105 2,5 nmol
(50 l)
22,00    Add this product to your notepad  
A1045
F4 reverse primer
(for integration diagnostics of all 'F4' expression vectors with 5'utr camBA)
PM-106 2,5 nmol
(50 l)
22,00    Add this product to your notepad  
A708
ble forward primer
(for integration diagnostics of all ble expression vectors)
PM-107 2,5 nmol
(50 l)
22,00    Add this product to your notepad  
A1432
neo forward primer
(for integration diagnostics of all neo expression vectors)
PM-108 2,5 nmol
(50 l)
22,00    Add this product to your notepad  
A3804
hyg forward primer
(for integration diagnostics of all hyg expression vectors)
PM-109 2,5 nmol
(50 l)
22,00    Add this product to your notepad  
P1442
Insert sequencing forward primer
(for all 'AP' expression vectors with 5'utr aprt)
PM-110 2,5 nmol
(50 l)
22,00    Add this product to your notepad  
A1715
AP reverse primer
(for integration diagnostics of all 'AP' expression vectors with 5'utr aprt)
PM-111 2,5 nmol
(50 l)
22,00    Add this product to your notepad  
E158
tub forward primer
(for integration diagnostics of all β-tub integration vectors)
PM-112 2,5 nmol
(50 l)
22,00    Add this product to your notepad  
E159
tub reverse primer
(for integration diagnostics of all β-tub integration vectors)
PM-113 2,5 nmol
(50 l)
22,00    Add this product to your notepad  
A1304
odc forward primer
(for integration diagnostics of all odc integration vectors)
PM-114 2,5 nmol
(50 l)
22,00    Add this product to your notepad  
P1510
odc reverse primer
(for integration diagnostics of all odc integration vectors)
PM-115 2,5 nmol
(50 l)
22,00    Add this product to your notepad  
H4132
Insert sequencing forward primer
(for pLEXSY_invitro-1 expression vector)
PM-116 2,5 nmol
(50 l)
22,00    Add this product to your notepad  
H4134
Forward primer for overlap extension PCR
(for LEXSY cell-free protein expression kit)
PM-117 0,5 nmol
(50 l)
22,00    Add this product to your notepad  
H4662
neo forward primer
(for integration diagnostics of all neo expression vectors)
PM-118 2,5 nmol
(50 l)
22,00    Add this product to your notepad  

 
 


Please contact expression@jenabioscience.com with questions or inquiries.