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"T" Enzymes - Tth111 I| Tth111 I Isoschizomers: Asp I, PflF I, Psy I, Tel I |
GACN↓NNGTC | ||||
| Product | Cat. No. | Amount | Price (EUR) | Buy / Note | |
|---|---|---|---|---|---|
| S pack | EN-E2420-01 | 1000 Units | 45,00 | 
 |
 |
| L pack | EN-E2420-02 | 5000 Units | 180,00 |
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Recognition Sequence:
Source: Thermus thermophilus 111.
Buffer supplied: 10x Reaction Buffer B1.
Storage conditions: 10 mM Tris-HCl (pH 7.5 at 25°C), 200 mM KCl,
0.1 mM EDTA, 1 Mm dithiothreitol, 200 µg/ml BSA and 50 % [v/v] glycerol.
Store at -20°C.
Reaction conditions: 10 mM Tris-HCl (pH 7.9 at 25°C), 10 mM MgCl2, 1 mM dithiothreitol and 100 µg/ml BSA.
Incubate at 65°C.
5
'
-
G
A
C
N
|
N
N
G
T
C
-
3
'
3
'
-
C
T
G
N
N
|
N
C
A
G
-
5
'
Source: Thermus thermophilus 111.
Buffer supplied: 10x Reaction Buffer B1.
Storage conditions: 10 mM Tris-HCl (pH 7.5 at 25°C), 200 mM KCl,
0.1 mM EDTA, 1 Mm dithiothreitol, 200 µg/ml BSA and 50 % [v/v] glycerol.
Store at -20°C.
Reaction conditions: 10 mM Tris-HCl (pH 7.9 at 25°C), 10 mM MgCl2, 1 mM dithiothreitol and 100 µg/ml BSA.
Incubate at 65°C.
Heat inactivation: Not applicable for this enzyme.
DNA Methylation:
No Inhibition: dam, dcm, EcoKI, CpG.
Unit definition: One unit is the amount of enzyme required to completely digest 1 µg of Ad-2 DNA in 1 hour in a total reaction volume of 50 µl. Enzyme activity was determined in the recommended reaction buffer.
DNA Methylation:
No Inhibition: dam, dcm, EcoKI, CpG.
Unit definition: One unit is the amount of enzyme required to completely digest 1 µg of Ad-2 DNA in 1 hour in a total reaction volume of 50 µl. Enzyme activity was determined in the recommended reaction buffer.
Please contact enzymes@jenabioscience.com for more information.