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"S" Enzymes - Spe I| Spe I Isoschizomers: Ahl I, Bcu I |
A↓CTAGT | ||||
| Product | Cat. No. | Amount | Price (EUR) | Buy / Note | |
|---|---|---|---|---|---|
| S pack | EN-E2398-01 | 500 Units | 41,00 | 
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| L pack | EN-E2398-02 | 2500 Units | 162,00 |
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Recognition Sequence:
Source: Sphaerotilus species.
Buffer supplied: 10x Reaction Buffer B2.
Storage conditions: 10 mM Tris-HCl (pH 7.5 at 22°C), 50 mM KCl,
0.1 mM EDTA, 1 mM dithiothreitol, 100 µg/ml BSA and 50 % [v/v] glycerol.
Store at -20°C.
Reaction conditions: 10 mM Tris-HCl (pH 7.9 at 25°C), 10 mM MgCl2, 50 mM NaCl, 1 mM dithiothreitol and 100 µg/ml BSA.
Incubate at 37°C.
5
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A
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C
T
A
G
T
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T
G
A
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C
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A
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Source: Sphaerotilus species.
Buffer supplied: 10x Reaction Buffer B2.
Storage conditions: 10 mM Tris-HCl (pH 7.5 at 22°C), 50 mM KCl,
0.1 mM EDTA, 1 mM dithiothreitol, 100 µg/ml BSA and 50 % [v/v] glycerol.
Store at -20°C.
Reaction conditions: 10 mM Tris-HCl (pH 7.9 at 25°C), 10 mM MgCl2, 50 mM NaCl, 1 mM dithiothreitol and 100 µg/ml BSA.
Incubate at 37°C.
Heat inactivation: 20 minutes at 65°C.
DNA Methylation:
No inhibition: dam, dcm, CpG.
Potential Inhibition: EcoKI.
Unit definition: One unit is the amount of enzyme required to completely digest 1 µg of Ad-2 DNA in 1 hour in a total reaction volume of 50 µl. Enzyme activity was determined in the recommended reaction buffer.
DNA Methylation:
No inhibition: dam, dcm, CpG.
Potential Inhibition: EcoKI.
Unit definition: One unit is the amount of enzyme required to completely digest 1 µg of Ad-2 DNA in 1 hour in a total reaction volume of 50 µl. Enzyme activity was determined in the recommended reaction buffer.
Please contact enzymes@jenabioscience.com for more information.