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"B" Enzymes - BstX I| BstX I | CCANNNNN↓NTGG | ||||
| Product | Cat. No. | Amount | Price (EUR) | Buy / Note | |
|---|---|---|---|---|---|
| S pack | EN-E2118-01 | 1000 Units | 41,00 | 
 |
 |
| L pack | EN-E2118-02 | 5000 Units | 162,00 |
|
|
Recognition Sequence:
Source: Bacillus stearothermophilus
Buffer supplied: 10x Reaction Buffer B3.
Storage conditions: 10 mM Tris-HCl (pH 7.5 at 22°C), 50 mM KCl,
0.1 mM EDTA, 1 mM dithiothreitol, 100 µg/ml BSA and 50 % [v/v] glycerol.
Store at -20°C.
Reaction conditions: 50 mM Tris-HCl (pH 7.9 at 25°C),
10 mM MgCl2, 100 mM NaCl, 1 mM dithiothreitol and 100 µg/ml BSA.
Incubate at 50°C.
5
'
-
C
C
A
N
N
N
N
N
|
N
T
G
G
-
3
'
3
'
-
G
G
T
N
|
N
N
N
N
N
A
C
C
-
5
'
Source: Bacillus stearothermophilus
Buffer supplied: 10x Reaction Buffer B3.
Storage conditions: 10 mM Tris-HCl (pH 7.5 at 22°C), 50 mM KCl,
0.1 mM EDTA, 1 mM dithiothreitol, 100 µg/ml BSA and 50 % [v/v] glycerol.
Store at -20°C.
Reaction conditions: 50 mM Tris-HCl (pH 7.9 at 25°C),
10 mM MgCl2, 100 mM NaCl, 1 mM dithiothreitol and 100 µg/ml BSA.
Incubate at 50°C.
Heat inactivation: 20 minutes at 65°C.
DNA Methylation:
No Inhibition: dam, EcoKI, CpG.
Potential Inhibition: dcm.
Unit definition: One unit is the amount of enzyme required to completely digest 1 µg of Lambda DNA in 1 hour in a total reaction volume of 50 µl. Enzyme activity was determined in the recommended reaction buffer.
DNA Methylation:
No Inhibition: dam, EcoKI, CpG.
Potential Inhibition: dcm.
Unit definition: One unit is the amount of enzyme required to completely digest 1 µg of Lambda DNA in 1 hour in a total reaction volume of 50 µl. Enzyme activity was determined in the recommended reaction buffer.
Please contact enzymes@jenabioscience.com for more information.