Recognition Sequence:


Source: Bacillus aneurinolyticus.

Buffer supplied: 10x Reaction Buffer B5.

Storage conditions: 10 mM Tris-HCl (pH 7.4 at 22°C), 50 mM NaCl,
0.1 mM EDTA, 1 mM dithiothreitol, 0.01% Triton™X-100, 200 µg/ml BSA and 50 % [v/v] glycerol.
Store at -20°C.

Reaction conditions: 20 mM Tris-acetate (pH 7.9 at 25°C), 10 mM magnesium acetate, 50 mM potassium acetate, 1 mM dithiothreitol and 100 µg/ml BSA.
Incubate at 37°C.

Heat inactivation: 20 minutes at 65°C.

DNA Methylation:
No inhibition: dam, EcoKI, dcm, CpG.

Unit definition: One unit is the amount of enzyme required to completely digest 1 µg of Lambda DNA in 1 hour in a total reaction volume of 50 µl. Enzyme activity was determined in the recommended reaction buffer.