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"A" Enzymes - Ava I| Ava I Isoschizomers: Ama87 I, BsiHKC I, BsoB I, Eco88 I, Nsp III Neoschizomers:BmeT110 I |
C↓YCGRG | ||||
| Product | Cat. No. | Amount | Price (EUR) | Buy / Note | |
|---|---|---|---|---|---|
| S pack | EN-E2006-01 | 2000 Units | 36,00 | 
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| L pack | EN-E2006-02 | 10000 Units | 143,00 |
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Recognition Sequence:
Source: Anabaena variabilis.
Buffer supplied: 10x Reaction Buffer B2.
Storage conditions: 10 mM Tris-HCl (pH 7.4 at 22°C), 50 mM KCl,
1 mM dithiothreitol, 0.1 mM EDTA and 50 % [v/v] glycerol.
Store at -20°C.
Reaction conditions: 10 mM Tris-HCl (pH 7.9 at 25°C), 10 mM MgCl2, 50 mM NaCl, 1 mM dithiothreitol and 100 µg/ml BSA.
Incubate at 37°C
5
'
-
C
|
Y
C
G
R
G
-
3
'
3
'
-
G
R
G
C
Y
|
C
-
5
'
Source: Anabaena variabilis.
Buffer supplied: 10x Reaction Buffer B2.
Storage conditions: 10 mM Tris-HCl (pH 7.4 at 22°C), 50 mM KCl,
1 mM dithiothreitol, 0.1 mM EDTA and 50 % [v/v] glycerol.
Store at -20°C.
Reaction conditions: 10 mM Tris-HCl (pH 7.9 at 25°C), 10 mM MgCl2, 50 mM NaCl, 1 mM dithiothreitol and 100 µg/ml BSA.
Incubate at 37°C
Heat inactivation: 20 minutes at 65°C.
DNA Methylation:
No Inhibition: dam, dcm, EcoKI.
Inhibition (Blocked): CpG.
Unit definition: One unit is the amount of enzyme required to completely digest 1 µg of Lambda DNA in 1 hour in a total reaction volume of 50 µl. Enzyme activity was determined in the recommended reaction buffer.
DNA Methylation:
No Inhibition: dam, dcm, EcoKI.
Inhibition (Blocked): CpG.
Unit definition: One unit is the amount of enzyme required to completely digest 1 µg of Lambda DNA in 1 hour in a total reaction volume of 50 µl. Enzyme activity was determined in the recommended reaction buffer.
Please contact enzymes@jenabioscience.com for more information.