Catalog JBScreen Family - Pi-PEG ScreenJBScreen Family - Pi-PEG Screen
 
 
JBScreen Classic   |   JBScreen Basic   |   JBScreen Membrane   |   JBScreen Kinase   |   JBScreen Nuc-Pro   |   JBScreen PEG/Salt   |   JBScreen Pentaerythritol   |   JBScreen PACT++   |   JBScreen JCSG++   |   Pi-minimal Screen   |   Pi-PEG Screen   |   JBScreen Wizard
 
Pi minimal Screen
Application

Efficient crystallization screening of soluble proteins and an integral membrane proteins

Format

Bulk 96 screening solutions in 10 ml aliquots
HTS 96 screening solutions delivered in a deep-well block, 1.7 ml per well
 
The Pi-PEG Screen was developed at the MRC Laboratory of Molecular Biology (Cambridge, UK). The strategy employed is based on incomplete factorial design [1]. It has been designed for efficient crystallization screening of integral membrane proteins benefiting from the experience of membrane protein crystallization at the MRC.

Like for the Pi-minimal screen, the unique formulation was generated following a strategy named Pi sampling [1] in order to create novel combinations of precipitants, buffers and additives across a standard 96-condition plate layout. Thus, the diversity amongst the crystallization conditions is ideal for initial screening.

The efficiency of the Pi-PEG screen was demonstrated by the crystallization of a G-protein coupled receptor (GPCR) when quality crystals could not be produced with other commercially available screens [1].

The Pi-PEG Screen includes various polyethylene glycol mixtures, additives and buffers covering a pH range from 4.0 9.5 and hence it is also appropriate for soluble proteins.
 
 


Individual Conditions of all screens are available in 10 ml as well as 100 ml volumes. Please follow this link.
 
 
 

Product Cat. No. Amount Price (EUR) Buy / Note Downloads
Pi-PEG Screen CS-128 4 x 24 solutions
(10 ml each)
450,00    Add this product to your notepad      
Pi-PEG Screen HTS CS-212L 96 solutions
(1,7 ml each)
225,00    Add this product to your notepad      

Reference

[1] Gorrec et al. (2011) Pi sampling: a methodical and flexible approach to initial macromolecular crystallization screening. Acta Cryst. D67:463.

Available online at http://journals.iucr.org/d/issues/2011/05/00/bw5391/index.html
 


Please contact xtals@jenabioscience.com with questions or inquiries.