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Modified Nucleotides - Puromycin DerivativesPuromycin conjugates for in vitro and in vivo labeling of proteins
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Traditionally, the protein(s) under investigation need to be expressed, purified and subsequently labeled by chemical methods which is often time consuming and cumbersome.
A new and significantly faster approach, using biotin- and dye-labeled puromycin conjugates, can be applied to in vitro (using established cell free expression systems)[6-7] as well as in vivo labeling (protein expression in cells)[8].
A labeled puromycin analog is incorporated into the C-terminus during protein synthesis on the ribosome to yield the corresponding labeled full-length protein. The synchronization of protein expression and labeling will eliminate the need for subsequent protein purification and chemical labeling.
| Biotin-dC-puromycin | ||||||
| Product | Cat. No. | Amount | Price (EUR) | Buy / Note | ||
|---|---|---|---|---|---|---|
| S pack | NU-925-BIO-S | 100 µl (0.1 mM) |
107,37 | 
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|
| L pack | NU-925-BIO-L | 500 µl (0.1 mM) |
314,50 |
|
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| 6-FAM-dC-puromycin | ||||||
| Product | Cat. No. | Amount | Price (EUR) | Buy / Note | ||
|---|---|---|---|---|---|---|
| S pack | NU-925-6FM-S | 100 µl (0.1 mM) |
107,37 |
|
|
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| L pack | NU-925-6FM-L | 500 µl (0.1 mM) |
314,50 |
|
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References:
[1] Coleman et al. (2004) High-throughput, fluorescence-based screening for soluble protein expression. J. Proteome Res. 3: 1024.
[2] Yan et al. (2003) Analysis of protein interactions using fluorescence technologies. Curr. Opin. Chem. Biol. 7: 635.
[3] Heyduk et al. (2002) Measuring protein conformational changes by FRET/LRET. Curr. Opin. Biotechnol. 13: 292.
[4] Hultschig et al. (2006) Recent advances of protein microarrays. Curr. Opin. Chem. Biol. 10: 4.
[5] Tomizaki et al. (2005) Protein-detecting microarrays: current accomplishments and requirements. ChemBioChem 6: 782.
[6] Nemoto et al. (1999) Fluorescence labeling of the C-terminus of proteins with a puromycin analogue in cell-free translation systems. FEBS Lett. 462 (1-2): 43.
[7] Kawahashi et al. (2007) High-throughput fluorescence labelling of full-length cDNA products based on a reconstituted translation system. J. Biochem. 141 (1): 19.
[8] Stark et al. (2004) A general approach to detect protein expression in vivo using fluorescent puromycin conjugates. Chem. Biol. 11 (7): 999.
[1] Coleman et al. (2004) High-throughput, fluorescence-based screening for soluble protein expression. J. Proteome Res. 3: 1024.
[2] Yan et al. (2003) Analysis of protein interactions using fluorescence technologies. Curr. Opin. Chem. Biol. 7: 635.
[3] Heyduk et al. (2002) Measuring protein conformational changes by FRET/LRET. Curr. Opin. Biotechnol. 13: 292.
[4] Hultschig et al. (2006) Recent advances of protein microarrays. Curr. Opin. Chem. Biol. 10: 4.
[5] Tomizaki et al. (2005) Protein-detecting microarrays: current accomplishments and requirements. ChemBioChem 6: 782.
[6] Nemoto et al. (1999) Fluorescence labeling of the C-terminus of proteins with a puromycin analogue in cell-free translation systems. FEBS Lett. 462 (1-2): 43.
[7] Kawahashi et al. (2007) High-throughput fluorescence labelling of full-length cDNA products based on a reconstituted translation system. J. Biochem. 141 (1): 19.
[8] Stark et al. (2004) A general approach to detect protein expression in vivo using fluorescent puromycin conjugates. Chem. Biol. 11 (7): 999.
Please contact nucleotides@jenabioscience.com with questions or inquiries.