RNA Labeling Kits
Jena Bioscience RNA Labeling Kits allow fluorescent labeling of RNA probes in less than two hours.
Thoroughly selected fluorophores are linked to UTP resulting in fluorescent nucleotides with superior properties such as high stability, high signal to noise ratios and high quantum yields. Our RNA Labeling Kits ensure efficient incorporation using T7 in vitro transcription. They are optimized for transcription efficiency and a high degree of labeling.
Typical RNA yields and degrees of labeling (DOL) are given in the table below. Experiments were performed using 500 ng template DNA (523 bp λ-DNA fragment containing a T7 promoter site) in a 20 µl reaction assay.
Thoroughly selected fluorophores are linked to UTP resulting in fluorescent nucleotides with superior properties such as high stability, high signal to noise ratios and high quantum yields. Our RNA Labeling Kits ensure efficient incorporation using T7 in vitro transcription. They are optimized for transcription efficiency and a high degree of labeling.
Typical RNA yields and degrees of labeling (DOL) are given in the table below. Experiments were performed using 500 ng template DNA (523 bp λ-DNA fragment containing a T7 promoter site) in a 20 µl reaction assay.
| Emission Color | Fluorophore | Ex/Em [nm] | Yield | DOL |
| green | Atto 488 | 501/523 | 2 µg | 16.5 |
| yellow-green | Atto 532 | 532/553 | 3 µg | 3.3 |
| yellow | Atto 550 | 554/576 | 1.5 µg | 2.9 |
| red | Atto 647N | 644/669 | 2 µg | 1.5 |
| far-red | Atto 680 | 680/700 | 2.5 µg | 2.2 |
Please contact info@jenabioscience.com with questions or inquiries.
