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Protein Prenylation - Lipid DonorsProtein Prenyl Transferases
| Lipid Donors
| FTase Substrates and Related Proteins
| GGTase-I Substrates and Related Proteins
| RabGGTase Substrates and Related Proteins
| Inhibitors
Innovative lipid donors have been developed for sensitive prenylation assays. NBD-FPP and NBD-GPP are a well-established fluorescent analogs of the natural lipid donors.
B-GPP is the first universal lipid donor for all prenylatable proteins, providing versatile prenylation assays with femtomolar detection sensitivity.
B-GPP is the first universal lipid donor for all prenylatable proteins, providing versatile prenylation assays with femtomolar detection sensitivity.
| Product | Cat. No. | Amount | Price (EUR) | Buy / Note | Downloads |
|---|---|---|---|---|---|
| B-GPP Biotin-labeled geranyl pyrophosphate, triethylammonium salt |
LI-015 | 20 µl | 180,00 | 
 |
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| NBD-FPP {3,7,11-trimethyl-12-(7-nitro-benzo[1,2,5]oxadiazo-4-ylamino) -dodeca-2,6,10-trien-1} pyrophosphate Fluorescent lipid donor for protein geranylgeranyltransferases (RabGGTase, GGTase-I) |
LI-013 | 20 µl | 180,00 |
|
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| NBD-GPP 3,7-dimethyl-8-(7-nitro-benzo[1,2,5]oxadiazol-4-ylamino) -octa-2,6-diene-1- pyrophosphate Fluorescent lipid donor for protein farnesyltransferase (FTase) |
LI-014 | 20 µl | 180,00 |
|
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| FPP Farnesyl pyrophosphate, ammonium salt Natural lipid donor for protein farnesyltransferase (FTase) |
LI-016 | 200 µg | 128,00 |
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| GGPP Geranylgeranyl pyrophosphate, ammonium salt Natural lipid donor for protein geranylgeranyltransferases (RabGGTase, GGTase-I) |
LI-017 | 200 µg | 128,00 |
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Selected References
- Nguyen et al. (2009) Analysis of the eukaryotic prenylome by isoprenoid affinity tagging. Nature Chemical Biology. 5 (4):227
- Wu et al. (2007) Synthesis of a fluorescent analogue of geranylgeranyl pyrophosphate and its use in a high-throughput fluorometric assay for Rab geranylgeranyltransferase. Nat. Protoc. 2 (11):2704
- Dursina et al. (2006) Identification and specificity profiling of protein prenyltransferase inhibitors using new fluorescent phosphoisoprenoids. J. Am. Chem. Soc. 128 (9):2822
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