Catalog Protein Prenylation - Protein Prenyl TransferasesProtein Prenylation - Protein Prenyl Transferases
 
 
Protein Prenyl Transferases   |   Lipid Donors   |   FTase Substrates and Related Proteins   |   GGTase-I Substrates and Related Proteins   |   RabGGTase Substrates and Related Proteins   |   Inhibitors
 
FTase and GGTase-I are designated CaaX prenyltransferases, since they recognize their protein substrates by the so-called CaaX-box. Both enzymes share a common α subunit (48 kDa) and a homologous β subunit (46 kDa in FTase, 42 kDa in GGTase-I). GGTase-II or RabGGTase differs from FTase and GGTase-I, both structurally and functionally. The enzyme attaches geranylgeranyl groups to two C-terminal cysteines of Rab proteins that terminate in CC or CXC motifs.

Engineered mutants of FTase (Cat.# PR-952 and PR-953) and GGTase-I (Cat.# PR-954) in combination with our innovative and universal lipid donor B-GPP (Cat.# LI-015) allow the detection of femtomolar amounts of prenylatable proteins in eukaryotic cells and tissues and thereby offer a new generation of prenylation studies.
 

Product Accession No. Cat. No. Amount Price (EUR) Buy / Note Downloads
FTase
Protein farnesyltransferase, α- and β-subunit
rat, recombinant, E. coli
Q04631 / Q02293 PR-102 50 g 180,00    Add this product to your notepad  
FTaseGST
Protein farnesyltransferase, α- and β-subunit
rat, recombinant, E. coli
Q04631 / Q02293 PR-361 50 g 180,00    Add this product to your notepad  
FTaseW102T_Y154TGST
Protein farnesyltransferase mutant, α- and β-subunit
rat, recombinant, E. coli
PR-952 20 g 245,00    Add this product to your notepad  
FTaseW102T_Y154T_Y205TGST
Protein farnesyltransferase mutant, α- and β-subunit
rat, recombinant, E. coli
PR-953 20 g 245,00    Add this product to your notepad  
GGTase-I
Protein geranylgeranyltransferase type I
rat, recombinant, E. coli
NM_031082 PR-101 50 g 180,00    Add this product to your notepad  
GGTase-IGST
Protein geranylgeranyltransferase type I
rat, recombinant, E. coli
NM_031082 PR-360 50 g 180,00    Add this product to your notepad  
GGTase-IF53Y_Y126TGST
Protein geranylgeranyltransferase type I mutant, α- and β-subunit
rat, recombinant, E. coli
PR-954 20 g 245,00    Add this product to your notepad  
GGTase-II (RabGGTase)
Protein geranylgeranyltransferase type II, α- and β-subunit
rat, recombinant, E. coli
Q08602 / Q08603 PR-103 50 g 227,00    Add this product to your notepad  
REP-1 His
Rab Escort Protein
fish, recombinant, E. coli
PR-105 50 g 180,00    Add this product to your notepad  
REP-2His
Rab Escort Protein
human, recombinant, Saccharomyces cerevisiae
NM_001821 PR-104 50 g 180,00    Add this product to your notepad  

Selected References

  • Hougland et al. (2009) Getting a handle on protein prenylation. Nat. Chem. Biol. 5 (4):197
  • Nguyen et al. (2009) Analysis of the eukaryotic prenylome by isoprenoid affinity tagging. Nat. Chem. Biol. 5 (4):227
  • Lane et al. (2006) Structural biology of protein farnesyltransferase and geranylgeranyltransferase type I. J. Lipid Res. 47:681
  • Casey et al. (1996) Protein Prenyltransferases. J. Biol. Chem. 271 (10):5289
 
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