Lyophilisates – Preloaded and Stable at Room Temperature

Ready-to-Use Lyophilisates are deliverd in PCR reaction tube strips or 96-well plates preloaded with a complete master mix in a dry, room temperature stable format. The lyophilisates combine highest performance with convenience of use and stability. There is no need for freezing, thawing or pipetting on ice. The few remaining pipetting steps minimize the risk of errors or contaminations. Each vial contains hot-start polymerase, dNTPs and reaction buffer required for a 20 μl PCR assay. Simply fill up the vials with template DNA, primer and PCR-grade water and run the PCR in a thermocycler as usual.
Hot Start Master Lyophilisate requires no prolonged heating or denaturing step. The polymerase inhibiting ligand is quickly released at the increased temperature of thermal cycling.

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Ready-to-Use Lyophilisates containing custom primers are custom made master mix lyophilisates comprising primers, polymerase, dNTPs and reaction buffer. Provide us with primer sequence and we deliver preloaded PCR tubes and plates ready-made for your special application. The only thing you still have to do is adding template DNA, filling up with water and starting the cycler!